Endocrine disrupting effects of zearalenone, alpha- and beta-zearalenol at the level of nuclear receptor binding and steroidogenesis

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dc.contributor.authorFrizzell, C.
dc.contributor.authorNdossi, D.
dc.contributor.authorVerhaegenb, S.
dc.contributor.authorDahlb, E.
dc.contributor.authorEriksend, G.
dc.contributor.authorSørlie, M.
dc.contributor.authorRopstadb, E.
dc.contributor.authorMullerf, M.
dc.contributor.authorElliott, C.
dc.contributor.authorConnollya, L.
dc.date.accessioned2020-04-15T05:09:27Z
dc.date.available2020-04-15T05:09:27Z
dc.date.issued2011-07-23
dc.descriptionToxicology Letters, 2011; 206: 210– 217en_US
dc.description.abstractThe mycotoxin zearalenone (ZEN) is a secondary metabolite of fungi which is produced by certain species of the genus Fusarium and can occur in cereals and other plant products. Reporter gene assays incorporating natural steroid receptors and the H295R steroidogenesis assay have been implemented to assess the endocrine disrupting activity of ZEN and its metabolites -zearalenol (-ZOL) and -zearalenol ( -ZOL). -ZOL exhibited the strongest estrogenic potency (EC50 0.022 ± 0.001 nM), slightly less potent than 17- estradiol (EC50 0.015 ± 0.002 nM). ZEN was ∼70 times less potent than -ZOL and twice as potent as -ZOL. Binding of progesterone to the progestagen receptor was shown to be synergistically increased in the presence of ZEN, -ZOL or -ZOL. ZEN, -ZOL or -ZOL increased production of progesterone, estradiol, testosterone and cortisol hormones in the H295R steroidogenesis assay, with peak productions at 10 M. At 100 M, cell viability decreased and levels of hormones were significantly reduced except for progesterone. ZOL increased estradiol concentrations more than -ZOL or ZEN, with a maximum effect at 10 M, with -ZOL (562 ± 59 pg/ml) > -ZOL (494 ± 60 pg/ml) > ZEN (375 ± 43 pg/ml). The results indicate that ZEN and its metabolites can act as potential endocrine disruptors at the level of nuclear receptor signalling and by altering hormone production.en_US
dc.identifier.issn210– 217
dc.identifier.urihttps://www.suaire.sua.ac.tz/handle/123456789/3008
dc.language.isoenen_US
dc.publisherElsevier Ireland Ltd.en_US
dc.subjectZearalenoneen_US
dc.subjectReporter gene assayen_US
dc.subjectH295Ren_US
dc.subjectSterodogenesisen_US
dc.subjectEndocrine disruptoren_US
dc.titleEndocrine disrupting effects of zearalenone, alpha- and beta-zearalenol at the level of nuclear receptor binding and steroidogenesisen_US
dc.typeArticleen_US
dc.urlhttps://www.researchgate.net/publication/51534427_Endocrine_disrupting_effects_of_zearalenone_alpha-_and_beta-zearalenol_at_the_level_of_nuclear_receptor_binding_and_steroidogenesisen_US

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