Epidemiological study of infectious bursal disease virus in selected districts of the Copperbelt province in Zambia

dc.contributor.authorChawinga, Kenneth
dc.date.accessioned2017-05-29T16:05:29Z
dc.date.available2017-05-29T16:05:29Z
dc.date.issued2016
dc.descriptionA DISERTATION SUBMITTED IN PARTIAL FUFILMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE IN APPLIED MICROBIOLOGY OF THE SOKOINE UNIVERSITY OF AGRICULTURE. MOROGORO, TANZANIA. 2016en_US
dc.description.abstractInfectious bursal disease (IBD) is a highly contagious disease of young chickens between 3 and 6 weeks of age. It is caused by infectious bursal disease virus(IBDV) which occursworldwide affecting livelihoods of resource - compromised poor communities. In Zambia, there is scantily documented information on the epidemiology of IBD. In-depth knowledge on the epidemiology of IBD is needed for effective control measures. This study aimed at molecular detection of circulating IBDV strains, andknowledge assessment of farmers about the disease in Ndola, Kitwe, Kalulushi, Luanshya and Mufulira districts of the Copperbelt province. A cross-sectional purposive study was carried out in the Copperbelt province from February to March, 2015 to determine the occurrence of IBD. The identification of IBDV was done by reverse transcription polymerase chain reaction (RT-PCR) targeting the hypervariable domain (VP2-HVR). A semi-structured questionnaire was administered to 77 respondents who presented poultry related cases to clinics in the selected districts and the information collected was analyzed by statistical package for social scientists (SPSS). A total of 30 bursa of Fabricius samples from young chickens that presented with clinical signs suggestive of IBD were examined. The RTPCR results revealed two positive samples for IBDV VP2-HVR domain. Questionnaire study revealed that 70.0% (n=10) of the respondents did not know what disinfectant to use; 75.0% (n=57) felt assigning more than one individual to a flock of chickens did not compromise biosecurity whereas 20.3% (n=15) knew the important clinical signs of IBD, compared to 60.1% (n=46) and 70.7% (n=54) that knew clinical signs related to chronicrespiratory disease and ND respectively. Ninety six percent (n= 73) adhered to vaccine cold chain practices. These findings indicate that IBD viruses circulated in the Copperbelt province and chicken farmers had low awareness of IBD and respective disease control measures. Further studies to characterize the circulating IBD viruses to unravel more information for the rational IBD control strategy in Zambia are required.en_US
dc.identifier.urihttps://www.suaire.sua.ac.tz/handle/123456789/1541
dc.language.isoenen_US
dc.publisherSokoine University of Agricultureen_US
dc.subjectEpidemiological studyen_US
dc.subjectInfectious bursal disease virusen_US
dc.subjectCopperbelt provinceen_US
dc.subjectZambiaen_US
dc.subjectEpidemiologyen_US
dc.titleEpidemiological study of infectious bursal disease virus in selected districts of the Copperbelt province in Zambiaen_US
dc.typeThesisen_US

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