Insights into potential pathogenesis mechanisms associated with Campylobacter jejuni-induced abortion in ewes

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dc.contributor.authorSanad, Y. M.
dc.contributor.authorJung, K.
dc.contributor.authorKashoma, I.
dc.contributor.authorZhang, X.
dc.contributor.authorKassem, I. I.
dc.contributor.authorSaif, Y. M.
dc.contributor.authorRajashekara, G.
dc.date.accessioned2018-06-18T14:20:58Z
dc.date.available2018-06-18T14:20:58Z
dc.date.issued2014-11-25
dc.description.abstractBackground: Campylobacter jejuni is commonly found in the gastrointestinal tract of many food-animals including sheep without causing visible clinical symptoms of disease. However, C. jejuni has been implicated in ovine abortion cases worldwide. Specifically, in the USA, the C. jejuni sheep abortion (SA) clone has been increasingly associated with sheep abortion. In vivo studies in sheep (the natural host) are needed to better characterize the virulence potential and pathogenesis of this clone. Results: Pregnant ewes intravenously (IV) or orally inoculated with ovine or bovine abortion-associated C. jejuni SA clones exhibited partial or complete uterine prolapse with retained placenta, and abortion or stillbirth, whereas delivery of healthy lambs occurred in pregnant ewes inoculated with C. jejuni 81–176 or in the uninfected group. In sheep inoculated with the SA clone, histopathological lesions including suppurative necrotizing placentitis and/or endometritis coincided with: 1) increased apoptotic death of trophoblasts, 2) increased expression of the host genes (e.g. genes encoding interleukin IL-6 and IL-15) related to cellular necrosis and pro-inflammatory responses in uterus, and 3) decreased expression of the genes encoding GATA binding protein 6, chordin, and insulin-like 3 (INSL3) that account for embryonic development in uterus. Immunohistochemistry revealed localization of bacterial antigens in trophoblasts lining the chorioallantoic membrane of ewes inoculated with the C. jejuni SA clone. Conclusions: The results showed that C. jejuni SA clones are capable of causing abortion or stillbirth in experimentally infected sheep. Furthermore, down- or up-regulation of specific genes in the uterus of infected pregnant ewes might implicate host genes in facilitating the disease progression. Since the C. jejuni SA strains share genotypic similarities with clones that have been isolated from human clinical cases of gastroenteritis, these strains might represent a potential public health risk.en_US
dc.identifier.urihttps://www.suaire.sua.ac.tz/handle/123456789/2391
dc.language.isoenen_US
dc.publisherBMC Veterinary Researchen_US
dc.subjectCampylobacter jejunien_US
dc.subjectSheep abortionen_US
dc.subjectPathogenesisen_US
dc.subjectPublic healthen_US
dc.subjectPregnant ewesen_US
dc.titleInsights into potential pathogenesis mechanisms associated with Campylobacter jejuni-induced abortion in ewesen_US
dc.typeArticleen_US

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