Occurrence, species distribution and antimicrobial resistance of thermophilic Campylobacter isolates from farm and laboratory animals in Morogoro, Tanzania
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Date
2014
Journal Title
Journal ISSN
Volume Title
Publisher
Veterinary World,
Abstract
Aim: To determine the carriage and antimicrobial resistance of Thermophilic Campylobacter species in the gastrointestinal
tracts of farm and laboratory animals in Morogoro, Tanzania
Materials and Methods: Faecal samples were collected from farm (n=244) and laboratory (n=466) animals and were
subjected to the Cape Town protocol for isolation of Campylobacter. Isolates were preliminarily identified based on
potassium hydroxide string and hippurate hydrolysis tests. Polymerase chain reaction (PCR) was employed for confirmation
of isolates.Antimicrobial resistance testing was done using disc diffusion method.
Results: Of the laboratory animals, 26.7% of guinea pigs (n=30) and 1.2% of rats (n=242) were colonized with
Campylobacter. Four isolates from guinea pigs were Campylobacter jejuni and the other four were Campylobacter coli. From
rats, two isolates were C. jejuni and one was C. coli. In farm animals thermophilic Campylobacter were detected from 31.6%
of sheep (n=57) and 60% horses (n=5). Of the isolates 12 (57%) were C. jejuni (10 from sheep and 2 from horses) and the
remaining were C. coli (8 from sheep and 1 from a horse). The isolates were frequently resistant to erythromycin, norfloxacin,
colistin sulphate and nalidixic acid; whereas low levels of resistance were observed for ciprofloxacin and gentamicin.
Conclusion: Our study reveals carriage of antimicrobial resistant thermophilic Campylobacter in the intestines of the study
animals. This highlights possibilities in involvement of these animals in the epidemiology of Campylobacter infections. Thus,
there is a need to consider these animal species when planning control measures for this zoonotic bacterium.
Description
Keywords
cape town protocol, farm animals, laboratory animals, Thermophilic Campylobacter