Detection of proteolysis in milk by pseudomonas fluorescens using urea PAGE method
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Date
2017-12-28
Journal Title
Journal ISSN
Volume Title
Publisher
Macrothink Institute
Abstract
Proteolysis of milk during storage by two strains of Pseudomonas NCIMB 702085 (414) and
NCIMB 701274 (416) was investigated using the Urea PAGE method. Pseudomonas
fluorescens enzymes were also extracted and purified by dialysis before inoculation into UHT
skim milk in an attempt to partially purify the enzyme. Results showed that dialysis removed
some peptides and amino acids which would interfere with the assay procedure. The method
also confirmed that Pseudomonas fluorescens NCIMB 701274 (416) was more proteolytic
than Pseudomonas NCIMB 702085 (414). Thus, Urea PAGE is a useful method for monitoring
proteolysis in milk by Pseudomonas fluorescens.
Description
Journal article
Keywords
Proteolysis, Pseudomonas fluorescens, Dialysis, Urea PAGE, UHT milk