Monitoring proteolysis in milk caused by pseudomonas fluorescens by the TNBS method
Loading...
Date
2017-11
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Pearl Research Journals
Abstract
The objective of this study was to monitor proteolysis of milk during storage at 20 and 4° C by two strains of
Pseudomonas fluorescens namely: NCIMB 702085 (416) and NCIMB 701274 (414) by the TNBS method. Since
commercial Pseudomonas fluorescens enzymes are not available, live Pseudomonas fluorescens
microorganisms were inoculated into milk followed by extraction and purification by dialysis. Bacterial
enzymes obtained were then inoculated into UHT skim milk. Non-inoculated milk was used as a control. Results
obtained showed that Pseudomonas fluorescens NCIMB 702085 was more proteolytic than Pseudomonas
fluorescens NCIMB 701274. It was concluded that the TNBS method proved useful in monitoring proteolysis in
milk by Pseudomonas fluorescens.
Description
Journal article
Keywords
Bacterial enzymes, Dialysis, Purification, UHT skim milk