Detection of tilapia lake virus (TiLV) infection by PCR in farmed and wild Nile tilapia (Oreochromis niloticus) from Lake Victoria
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Date
2018-01-02
Journal Title
Journal ISSN
Volume Title
Publisher
Wiley
Abstract
Tilapia lake virus disease (TiLVD) has emerged to be an important viral disease of
farmed Nile tilapia (Oreochromis niloticus) having the potential to impede expansion
Department of Biotechnical and Diagnostic
Sciences, College of Veterinary Medicine
Animal Resources and Biosecurity,
Makerere University, Kampala, Uganda of aquaculture production. There is a need for rapid diagnostic tools to identify
3 no difference in prevalence between farmed and wild fish
infected fish to limit the spread in individual farms. We report the first detection of
TiLV infection by PCR in farmed and wild Nile tilapia from Lake Victoria. There was
the 442 samples examined from 191 fish, 28 were positive for TiLV by PCR. In
terms of tissue distribution, the head kidney (7.69%, N = 65) and spleen (10.99%,
N = 191), samples had the highest prevalence (p < .0028) followed by heart samples
(3.45%, N = 29). Conversely, the prevalence was low in the liver (0.71%, N = 140)
and absent in brain samples (0.0%, N = 17), which have previously been shown to
be target organs during acute infections. Phylogenetic analysis showed homology
between our sequences and those from recent outbreaks in Israel and Thailand.
Correspondence
Hetron Mweemba Munang’andu, Faculty of
Veterinary Medicine and Biosciences,
Department of Basic Sciences and Aquatic
Medicine, Section of Aquatic Medicine and
Nutrition, Norwegian University of Life
Sciences, Oslo, Norway.
Email: hetroney.
mweemba.munangandu@nmbu.no
Given that these findings were based on nucleic acid detection by PCR, future stud-
ies should seek to isolate the virus from fish in Lake Victoria and show its ability to
cause disease and virulence in susceptible fish.
Description
Keywords
Lake Victoria, Nile tilapia, PCR, Phylogenetic, Surveillance, Tilapia lake virus