Sokoine University of Agriculture

Soil contamination by Taenia solium egg DNA in rural villages in Kongwa district, Tanzania

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dc.contributor.author Maganira, Justine Daudi
dc.contributor.author Kidima, Winifrida
dc.contributor.author Mwita, Chacha John
dc.contributor.author Halvarsson, Peter
dc.contributor.author Höglund, Johan
dc.date.accessioned 2021-08-06T05:20:20Z
dc.date.available 2021-08-06T05:20:20Z
dc.date.issued 2020
dc.identifier.citation Justine Daudi Maganira , Winifrida Kidima , Chacha John Mwita , Peter Halvarsson & Johan Höglund (2020) Soil contamination by Taenia solium egg DNA in rural villages in Kongwa district, Tanzania, Infection Ecology & Epidemiology, 10:1, 1772668, DOI: 10.1080/20008686.2020.1772668 en_US
dc.identifier.uri https://www.suaire.sua.ac.tz/handle/123456789/3835
dc.description Journal article en_US
dc.description.abstract The presence ofTaenia solium DNA from eggs in soils around the households in four Tanzanian villages in Kongwa district were analysed in relation to seasonal fluctuations and infection risk implications. A total of 192 pooled soil samples from five sampling points per household were examined by droplet digital Polymerase Chain Reaction (ddPCR) from 96 pigkeeping households both during the dry and rainy seasons. The pooled samples were first processed by a flotation-double sieving technique, followed by screening for worm DNA employing universal primers targeting the mitochondrial cytochrome c oxidase subunit I (cox1) gene of human taeniid species and some other helminths. All DNA positive samples were later confirmed by a specific ddPCR probe assay targeting the mitochondrial cox1 gene of T. solium. A total of 17.2% (n = 33) samples were positive with the universal ddPCR, whereas T. solium DNA was confirmed by the specific ddPCR only in 3.1% (n = 3) of the surveyed households. The detection of T. solium DNA in this study spells out a low risk of exposure to T. solium eggs from contaminated household soil. Based on our results, ddPCR seems to be a promising technology for screening T. solium eggs in soil. en_US
dc.language.iso en en_US
dc.publisher aylor & Francis Group en_US
dc.subject Taenia solium en_US
dc.subject Eggs en_US
dc.subject Soil contamination en_US
dc.subject ddPCR en_US
dc.subject Kongwa en_US
dc.subject Tanzania en_US
dc.title Soil contamination by Taenia solium egg DNA in rural villages in Kongwa district, Tanzania en_US
dc.type Article en_US
dc.url https://doi.org/10.1080/20008686.2020.1772668 en_US


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