Molecular detection and evolutionary characteristics of recently recovered serotypes a and o foot-and-mouth disease viruses in selected areas of Tanzania
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Date
2020
Authors
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Publisher
Sokoine University of Agriculture
Abstract
Foot-and-mouth disease (FMD) is a highly contagious disease that infects cloven hoofed
animals. FMD is caused by FMD virus (FMDV), a picornavirus with a positive sense
single stranded RNA genome of about 8.5 kb in size. FMD is endemic in East African
countries including Tanzania. The general objective of this study was to determine the
genetic characteristics of recently circulated serotypes O and A FMDV field strains in
selected regions of Tanzania in 2019. A total of 36 (four serotype O and 22 serotype A)
archived epithelial samples collected from cattle and pig in Kibaha, Morogoro,
Sengerema, Butiama and Musoma were used in this study. The laboratory analysis of the
samples was performed by reverse transcription polymerase chain reaction (RT-PCR)
targeting the 3D and VP1 coding regions, VP1 sequencing and phylogeny. The RT-PCR
results revealed that 72% of samples (n= 26) were positive for FMDV genome. Molecular
typing of the FMDV genome positive samples was achieved by using serotype specific
primers of which serotypes A were 85 % (n= 22) and O were 15 % (n= 4). Phylogenetic
reconstructions were determined by neighbour-joining methods. Phylogenetic analysis of
VP1 showed genetic diversity among the circulating viruses and their molecular
relatedness with previously recorded sequences from East Africa, particularly Kenya and
Tanzania. These findings indicate that the 2019 FMDV types A and O responsible for the
disease outbreaks in Tanzania were the East Africa 2 (EA-2) and genotype I (GI) which
showed a very closely evolutionary relatedness with previous strains collected in Kenya
and Tanzania. Further studies are required to design new vaccine strains so as to prevent
upcoming outbreaks.
Description
Masters Thesis
Keywords
Molecular, Foot-and-mouth, Tanzania, Viruses