Sokoine University of Agriculture

Effects of sucrose, boric acid, ph, and incubation Time on in vitro germination of pollen and tube growth of Chinese fir

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dc.contributor.author Fragallah, S. A. D. A.
dc.contributor.author Lin, S
dc.contributor.author Lin, N
dc.contributor.author Ligate, E J
dc.contributor.author Chen, Y
dc.date.accessioned 2021-01-26T06:57:39Z
dc.date.available 2021-01-26T06:57:39Z
dc.date.issued 2019
dc.identifier.uri http://www.suaire.sua.ac.tz/handle/123456789/3333
dc.description.abstract In vitro pollen germination provides a novel approach and strategy to accelerate genetic improvement of tree breeding. Studies about pollen germination and tube growth of Chinese fir are limited. Therefore, this study aimed to investigate the effects of sucrose, boric acid, pH, and time of incubation on pollen germination and tube growth. Pollen from 9 clones were selected. In vitro germination was performed in basic media as control, and in different concentrations of sucrose (0, 10 and 15%), boric acid (0.01, 0.1 and 0.2%), and pH levels (4.5, 5 and 7). Pollen germination rates and tube growth were recorded periodically at 1, 12, 24, and 48 h. The results showed that sucrose imposes significant effects on pollen germination and tube growth. The effects are most obvious at concentration of 15%. Boric acid significantly promoted germination and tube growth. The promotion was most notable in lower concentration of 0.01%. The media adjusted to pH 7.0 boosted the germination and pollen tube growth. The optimum time of incubation was 24 and 48 h for pollen germination and tube growth, respectively. Sucrose, pH, and time of incubation were positively correlated, whereas boric acid negatively correlated with pollen germination and tube growth. This study provided experimental evidences for selecting viable pollens for Chinese fir breeding. en_US
dc.language.iso en en_US
dc.publisher Forests en_US
dc.subject Chinese fir en_US
dc.subject Pollen germination en_US
dc.subject In vitro germination en_US
dc.subject Pollen grains en_US
dc.subject Pollen tube growth en_US
dc.title Effects of sucrose, boric acid, ph, and incubation Time on in vitro germination of pollen and tube growth of Chinese fir en_US
dc.title.alternative Cunnighamial Lanceolata en_US
dc.type Article en_US
dc.url www.mdpi.com/journal/forests en_US


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