Sokoine University of Agriculture

Phenotypic and genotypic characterization of salmonella enterica in captive wildlife and exotic animal species in Ohio, USA

Show simple item record Farias, L. F. P. Oliveira, C. J. B. Medardus, J. J. Molla, B. Z. Wolfe, B. A. Gebreyes, W. A. 2020-08-25T10:19:28Z 2020-08-25T10:19:28Z 2015
dc.description Zoonoses and Public Health, 2015, 62, pg, 438–444 en_US
dc.description.abstract The purpose of this study was to investigate the occurrence, antimicrobial resis- tance patterns, phenotypic and genotypic relatedness of Salmonella enterica recovered from captive wildlife host species and in the environment in Ohio, USA. A total of 319 samples including faecal (n = 225), feed (n = 38) and envi- ronmental (n = 56) were collected from 32 different wild and exotic animal spe- cies in captivity and their environment in Ohio. Salmonellae were isolated using conventional culture methods and tested for antimicrobial susceptibility with the Kirby–Bauer disc diffusion method. Salmonella isolates were serotyped, and genotyping was performed using the pulsed-field gel electrophoresis (PFGE). Sal- monella was detected in 56 of 225 (24.9%) faecal samples; six of 56 (10.7%) envi- ronmental samples and six of 38 (15.8%) feed samples. Salmonella was more commonly isolated in faecal samples from giraffes (78.2%; 36/46), cranes (75%; 3/4) and raccoons (75%; 3/4). Salmonella enterica serotypes of known public health significance including S. Typhimurium (64.3%), S. Newport (32.1%) and S. Heidelberg (5.3%) were identified. While the majority of the Salmonella iso- lates were pan-susceptible (88.2%; 60 of 68), multidrug-resistant strains including penta-resistant type, AmStTeKmGm (8.8%; six of 68) were detected. Genotypic diversity was found among S. Typhimurium isolates. The identification of clon- ally related Salmonella isolates from environment and faeces suggests that indirect transmission of Salmonella among hosts via environmental contamination is an important concern to workers, visitors and other wildlife. Results of this study show the diversity of Salmonella serovars and public health implications of human exposure from wildlife reservoirs. en_US
dc.language.iso en en_US
dc.publisher Blackwell Verlag GmbH en_US
dc.subject Salmonella enterica en_US
dc.subject Wild animals en_US
dc.subject Environmental contamination en_US
dc.subject Antimicrobial resistance en_US
dc.subject Genotyping en_US
dc.title Phenotypic and genotypic characterization of salmonella enterica in captive wildlife and exotic animal species in Ohio, USA en_US
dc.type Article en_US
dc.url en_US

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