Sokoine University of Agriculture

Mycobacterium genotypes in pulmonary tuberculosis infections and their detection by trained African giant pouched rats

Show simple item record

dc.contributor.author Mgode, G. F.
dc.contributor.author Cohen-Bacrie, S.
dc.contributor.author Bedotto, M.
dc.contributor.author Weetjens, B. J.
dc.contributor.author Cox, C.
dc.contributor.author Jubitana, M.
dc.contributor.author Kuipers, D.
dc.contributor.author Machang’u, R. S.
dc.contributor.author Kazwala, R.
dc.contributor.author Mfinanga, S.G.
dc.contributor.author Kaufmann, S. H. E.
dc.contributor.author Drancourt, M.
dc.date.accessioned 2020-06-05T08:41:20Z
dc.date.available 2020-06-05T08:41:20Z
dc.date.issued 2015
dc.identifier.uri http://www.suaire.sua.ac.tz/handle/123456789/3077
dc.description Article of Curr Microbiol (2015) 70:212–218 en_US
dc.description.abstract Tuberculosis (TB) diagnosis in low-income countries is mainly done by microscopy. Hence, little is known about the diversity of Mycobacterium spp. in TB infections. Different genotypes or lineages of Mycobacte- rium tuberculosis vary in virulence and induce different inflammatory and immune responses. Trained Cricetomys rats show a potential for rapid diagnosis of TB. They detect over 28 % of smear-negative, culture-positive TB. How- ever, it is unknown whether these rats can equally detect sputa from patients infected with different genotypes of M. tuberculosis. A 4-month prospective study on diversity of Mycobacterium spp. was conducted in Dar es Salaam, Tanzania. 252 sputa from 161 subjects were cultured on Lowenstein-Jensen medium and thereafter tested by rats. Mycobacterial isolates were subjected to molecular iden- tification and multispacer sequence typing (MST) to determine species and genotypes. A total of 34 Mycobac- terium spp. isolates consisting of 32 M. tuberculosis, 1 M. avium subsp. hominissuis and 1 M. intracellulare were obtained. MST analyses of 26 M. tuberculosis isolates yielded 10 distinct MST genotypes, including 3 new genotypes with two clusters of related patterns not grouped by geographic areas. Genotype MST-67, shared by one- third of M. tuberculosis isolates, was associated with the Mwananyamala clinic. This study shows that diverse M. tuberculosis genotypes (n = 10) occur in Dar es Salaam and trained rats detect 80 % of the genotypes. Sputa with two M. tuberculosis genotypes (20 %), M. avium homin- issuis and M. intracellulare were not detected. Therefore, rats detect sputa with different M. tuberculosis genotypes and can be used to detect TB in resource-poor countries. en_US
dc.language.iso en en_US
dc.publisher Springer Science+Business en_US
dc.subject Mycobacterium genotypes en_US
dc.subject Pulmonary tuberculosis infections en_US
dc.subject Trained African Giant en_US
dc.subject Pouched Rats en_US
dc.title Mycobacterium genotypes in pulmonary tuberculosis infections and their detection by trained African giant pouched rats en_US
dc.type Article en_US
dc.url https://www.researchgate.net/publication/266570397 en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search SUA IR


Browse

My Account