Sokoine University of Agriculture

Relative quantification of the proteomic changes associated with the mycotoxin zearalenone in the H295R steroidogenesis model

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dc.contributor.author Busk, Ø. L.
dc.contributor.author Ndossi, D.
dc.contributor.author Verhaegen, S.
dc.contributor.author Connolly, L.
dc.contributor.author Eriksen, G.
dc.contributor.author Ropstad, E.
dc.contributor.author Sørlie, M.
dc.date.accessioned 2020-04-01T11:28:34Z
dc.date.available 2020-04-01T11:28:34Z
dc.date.issued 2011
dc.identifier.uri http://www.suaire.sua.ac.tz/handle/123456789/2982
dc.description Toxicon 58 (2011) pp. 533–542 en_US
dc.description.abstract Zearalenone (ZEN) is a mycotoxin with endocrine disrupting effects having vast economic implications in e.g. pig farming. Structurally, ZEN resembles 17 b -estradiol, and thus is able to bind to estrogen receptors (ER) in target cells. Because of this, it is also classified as a non-steroidal estrogen, a phytoestrogen, a mycoestrogen, and a growth promoter. Quantitative proteomic analysis was undertaken using stable-isotope labeling by amino acids in cell culture (SILAC) upon exposure of the steroidogenesis cell model H295R with ZEN to elucidate its effect on protein regulation. ZEN significantly regulated 21 proteins, including proteins with known endocrine disrupting effects and several oncogenes. In addition, network analysis using Ingenuity Pathway Analysis showed that ZEN affected the oxidative phosphorylation pathway and the mitochondrial dysfunction pathway, both previously reported to be involved in endocrine dysfunction. en_US
dc.language.iso en en_US
dc.publisher Elsevier Ltd en_US
dc.subject Zearalenone en_US
dc.subject Mycotoxins en_US
dc.subject Quantitative proteomics en_US
dc.title Relative quantification of the proteomic changes associated with the mycotoxin zearalenone in the H295R steroidogenesis model en_US
dc.type Article en_US
dc.url www.elsevier.com/locate/toxicon en_US


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