Anti-Mycobacterial Activity on Middlebrook 7H10 Agar of Selected Congolese Medicinal Plants
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Date
2018-12-06
Journal Title
Journal ISSN
Volume Title
Publisher
American Institute of Science
Abstract
In recent years, Tuberculosis (TB) has re-emerged as a serious public health problem worldwide. The disease spreads more
easily in overcrowded settings and in conditions of malnutrition and poverty. The emergence of multidrug resistant and lengthy
therapy reduces the patient compliance which comprises TB control strategies. In the current study, the leaves of Terminalia
ivorensis, Carapa procera, Fagara macrophylla, Anacardium occidentale, Ficus spp. and Drepanoalpha® were extracted
using petroleum ether, ethyl acetate and methanol in order to assess their anti-mycobacterial activity against Mycobacterium
tuberculosis H37Rv and Mycobacterium tuberculosis spp. on Middlebrook 7H10 agar using a qualitative approach where the
activity was determined by the presence or the absence of growth on the plate. The phytochemical screening was used for the
identification of the major groups of compounds found in the extracts. The methanolic extract displayed a good activity on
both strains than the petroleum ether and ethyl acetate. The presence of alkaloids, flavonoids, tannins, saponins, anthocyanins,
quinones known to be of medicinal importance points out a possible source for anti-mycobacterial agents to address the
problem of multidrug resistance. The in vitro findings of this study provide a partial support for the use of these plants in the
management of various infectious diseases as lead to drug discovery and should be reiterated and recommended for a clinical
trial using an animal model.
Description
Bioscience and Bioengineering' 2018; 4 (4): 68-77
Keywords
Tuberculosis, Middlebrook 7H10 Agar, Anti-mycobacterial Activity, Phytochemical Screening, Medicinal Plants