Sokoine University of Agriculture

Molecular characterization of Mycobacterium tuberculosis isolates from Tanga, Tanzania: First insight of MIRU-VNTR and microarray-based spoligotyping in a high burden country

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dc.contributor.author Hoza, A. S.
dc.contributor.author Mfinanga, S. G. M.
dc.contributor.author Moser, I.
dc.contributor.author König, B.
dc.date.accessioned 2019-08-14T13:29:24Z
dc.date.available 2019-08-14T13:29:24Z
dc.date.issued 2016-02-13
dc.identifier.issn 1472-9792
dc.identifier.uri https://www.suaire.sua.ac.tz/handle/123456789/2908
dc.description Tuberculosis2016; Vol. 98: 116-124 en_US
dc.description.abstract Molecular typing of Mycobacterium tuberculosis(MTB) has greatly enhanced the understanding of the population structure of MTB isolates and epidemiology of tuberculosis (TB). To characterize prevalent genotypes of MTB, microarrays‑based spoligotyping and mycobacterial interspersed repetitive unit‑variable number of tandem repeats (MIRU‑VNTR) were applied on 80 isolates collected from primary health care facilities in Tanga, North‑eastern Tanzania. A total of 18 distinct spoligotypes were identified. The lineages by order of their predominance were EAI and CAS families (26.25%, 21 isolates each), LAM family and T super‑family (10%, 8 isolates each), MANU family (3.75%, 3 isolates), Beijing family (2.5%, 2 isolates) and S family (1.25%, 1 isolate). Overall, sixteen (20%) strains could not be allocated to any lineage according to the SITVIT_WEB database. The allelic diversity (h) for specific MIRU‑VNTR loci showed a considerable variation ranging from 0.826 of VNTR locus 3192 to 0.141 of VNTR locus 2059. The allelic diversity for 11 loci (VNTR 3192, 2996, 2165, 960, 4052, 424, 4156, 2531, 1644, 802 and 3690) exceeded 0.6, indicating highly discriminatory power. Seven loci (VNTR 2163b, 2401, 1955, 577, 4348, 2687 and 580) showed moderate discrimination (0.3 h 0.6), and three loci (VNTR3007, 154 and 2059) were less polymorphic. The present study suggests that the TB cases in Tanga might be caused by a diverse array of MTB strain families that may be indicative of a cosmopolitan population with frequent migration and travel. Microarray‑based spoligotyping and MIRU‑VNTR could be reliable tools in detecting different MTB genotypes in high burden settings. en_US
dc.language.iso en en_US
dc.publisher Elsevier Ltd en_US
dc.subject Microarray‑based spoligotyping en_US
dc.subject MIRU‑VNTR en_US
dc.subject Mycobacterium tuberculosis en_US
dc.subject Primary health care facilities en_US
dc.title Molecular characterization of Mycobacterium tuberculosis isolates from Tanga, Tanzania: First insight of MIRU-VNTR and microarray-based spoligotyping in a high burden country en_US
dc.type Article en_US


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