A simple and sensitive method for the detection of “Oxytetracycine” levels in ready-to-eat beef by liquid chromatography-mass spectrometry
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Date
2016-07
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African Journal of Pharmacy and Pharmacology
Abstract
Antimicrobial drug residues have emerged as one of the public health problems worldwide. In this
study, a modified sensitive liquid chromatography mass spectrometry (LC-MS) method to detect the
“Oxytetracycine” (OTC) levels in ready-to-eat beef meat in Tanzania was evaluated. Beef samples were
extracted in acetonitrile in ethylenediaminetetraacetic acid (EDTA) buffer (pH 4), followed by cleaning
up with Supelclean ENVI-carb active coal and a stream of nitrogen gas. The wavelength of the diode
array detector (DAD) was set at 275 and 355 nm. The detection limit of the method was calculated as
18.2 ng/g and the recovery rate of OTC was 78.6%. A total of 45 ready-to-eat beef meat samples were
analyzed, with 16 (35.5%) and 29 (64.5%) barbequed and boiled samples, respectively. Of the 45
samples, 35 (77.8%) samples had OTC residues while 9 (25.7%) samples had violative residue levels
above the maximum residue limits recommended by the Food and Agriculture Organization and the
World Health Organization. The highest concentration was 545.2 ng/g. Therefore, withdrawal period and
proper use of antibiotics for animal production should be of concern as consumers are at risk of
adverse effects due to consumption of unacceptable levels of drug residues and a risk of developing
microbial resistance. To the best knowledge of the authors, this is the first study to evaluate LC-MS method to detect the OTC levels in ready-to-eat beef meat in Tanzania
Description
African Journal of Pharmacy and Pharmacology, 2016; 10 (28): 571-579
Keywords
Oxytetracycline, High performance liquid chromatography, Mass spectrometry, Ready-to-eat beef meat, Residues