Sokoine University of Agriculture

Isolation and characterization of Escherichia coli from animals, humans, and environment

Show simple item record Lupindu, A. M. 2018-02-08T06:27:16Z 2018-02-08T06:27:16Z 2017-07
dc.description.abstract Working on a diverse species of bacteria that have hundreds of pathotypes representing hundreds of strains and many closely related family members is a challenge. Appropriate research design is required not only to achieve valid desired outcome but also to minimize the use of resources, including time to outcome and intervention. This chapter outlines basics of Escherichia coli isolation and characterization strategies that can assist in research designing that matches the set objectives. Types of samples to be collected, collection and storage strategies, and processing of samples are described. Different approaches to isolation, confirmation and concentration of various E. coli strains are summarized in this chapter. Characterization and typing of E. coli isolates by biochemical, serological, and molecular methods have been explained so that an appropriate choice is made to suite a specific E. coli strain/pathotype. Some clues on sample and isolate preservation for future use are outlined, and general precautions regarding E. coli handling are also presented to the researcher to avoid improper planning and execution of E. coli-related research. Given different options, the best E. coli research design, however, should try as much as possible to shorten the length of time to outcomes. en_US
dc.language.iso en en_US
dc.publisher INTECH- Open Science/ Open Minds en_US
dc.subject E. coli en_US
dc.subject β-glucuronidase en_US
dc.subject Enterobacteriaceae en_US
dc.subject Cryoprotectant en_US
dc.subject IMViC en_US
dc.title Isolation and characterization of Escherichia coli from animals, humans, and environment en_US
dc.type Article en_US
dc.url en_US

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