Sokoine University of Agriculture

Isolation and potential for transmission of mycobacterium bovis at human–livestock–wildlife interface of the Serengeti Ecosystem, Northern Tanzania

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dc.contributor.author Katale, B. Z.
dc.contributor.author Mbugi, E. V.
dc.contributor.author Siame, K. K.
dc.contributor.author Keyyu, J. D.
dc.contributor.author Kendall, S.
dc.contributor.author Kazwala, R. R.
dc.contributor.author Dockrell, H. M.
dc.contributor.author Fyumagwa, R. D.
dc.contributor.author Michel, A. L.
dc.contributor.author Rweyemamu, M
dc.contributor.author Streicher, E. M.
dc.contributor.author Warren, R. M.
dc.contributor.author Helden, P.
dc.contributor.author Matee, M. I.
dc.date.accessioned 2017-11-15T08:16:38Z
dc.date.available 2017-11-15T08:16:38Z
dc.date.issued 2015
dc.identifier.issn 815-825
dc.identifier.uri http://www.suaire.sua.ac.tz/handle/123456789/1807
dc.description Transboundary and Emerging Diseases, 64 (2017); 815-825 en_US
dc.description.abstract Mycobacterium bovis, the causative agent of bovine tuberculosis (bTB), is a multihost pathogen of public health and veterinary importance. We characterized the M. bovis isolated at the human– livestock–wildlife interface of the Serengeti ecosystem to determine the epidemiology and risk of crossspecies transmission between interacting hosts species. DNA was extracted from mycobacterial cultures obtained from sputum samples of 472 tuberculosis (TB) suspected patients and tissue samples from 606 livestock and wild animal species. M. bovis isolates were characterized using spoligotyping and Mycobacterial Interspersed Repetitive Units-Variable Tandem Repeats (MIRU-VNTR) on 24 loci. Only 5 M. bovis were isolated from the cultured samples. Spoligotyping results revealed that three M. bovis isolates from two buffaloes (Syncerus caffer) and 1 African civet (Civettictis civetta) belonged to SB0133 spoligotype. The two novel strains (AR1 and AR2) assigned as spoligotype SB2290 and SB2289, respectively, were identified from indigenous cattle (Bos indicus). No M. bovis was detected from patients with clinical signs consistent with TB. Of the 606 animal tissue specimens and sputa of 472 TBsuspected patients 43 (7.09%) and 12 (2.9%), respectively, yielded non-tuberculous mycobacteria (NTM), of which 20 isolates were M. intracellulare. No M. avium was identified. M. bovis isolates from wildlife had 45.2% and 96.8% spoligotype pattern agreement with AR1 and AR2 strains, respectively. This finding indicates that bTB infections in wild animals and cattle were epidemiologically related. Of the 24 MIRU-VNTR loci, QUB 11b showed the highest discrimination among the M. bovis strains. The novel strains obtained in this study have not been previously reported in the area, but no clear evidence for recent cross-species transmission of M. bovis was found between human, livestock and wild animals. en_US
dc.language.iso en en_US
dc.publisher PubMed en_US
dc.subject Mycobacterium bovis en_US
dc.subject MIRU-VNTR en_US
dc.subject Human-animal interface en_US
dc.subject Serengeti ecosystem en_US
dc.subject Spoligotype en_US
dc.title Isolation and potential for transmission of mycobacterium bovis at human–livestock–wildlife interface of the Serengeti Ecosystem, Northern Tanzania en_US
dc.type Article en_US
dc.url https://www.ncbi.nlm.nih.gov/pubmed/26563417 en_US


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