Genetic diversity among nine free range local chicken ecotypes in Tanzania based on microsatelliteDNA polymorphisms
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Date
2008-06
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Abstract
The genetic diversity among nine free-range local chicken ecoivpes identified in Tanzania
was evaluated using 20 polymorphic microsatelliies markers. The ecotypes were named
Ching'toekuie, Mbeya, Morogoro-medium, Kuchi, Singamagazi, N'zenzegere, Pemba,
Tunga, and Unguja. A manual microsatellite typing procedure was adopted. Of the 20
microsaielliie loci, 16 were amplified by PCR and were highly polymorphic. Four to 15
alleles per locus and four to seven alleles per ecotype were detected. Gene diversity was venj
high (62 to 79%). The shortest genetic distance as measured by Nei's standard genetic
distance (Ds) was between Kuchi and Unguja ecotypes (Ds distance = 0.0671) while the
White Leghorn and Unguja ecotype (Ds distance= 0.9149) presented the longest genetic
distance. A Neighbour-loining dendogram based on Ds distance showed the local chicken
ecotypes to assort into three clusters. The bootstrapping values within the dendogram
ranged from 41 to 98%. These results indicate that the free-range local chicken ecotypes to
exist in different genetic groups. The accurate genetic typing of this animal genetic resource
is important in making rational decisions on the utilisation of the chickens for economical
use without compromising the conservation of each unique ecotype adapted to a particular
eco-climaiic zone.
Description
Tanzania Journal of Agricultural Sciences 2008, Vol. 9(1) : 54 - 68
Keywords
Genetic diversity, Free-range local chicken ecotypes, Microsatellites, Animal genetic resourcesIntroduction