Sokoine University of Agriculture

Molecular epidemiology of Salmonella gallinarum in chickens in Tanzania

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dc.contributor.author Mdegela, R. H.
dc.contributor.author Yongolo, M
dc.contributor.author Minga, U.M
dc.contributor.author Olsen, J.E
dc.date.accessioned 2017-04-06T07:26:44Z
dc.date.available 2017-04-06T07:26:44Z
dc.date.issued 2010-06-17
dc.identifier.citation Robinson H. Mdegela , Mmeta G.S. Yongolo , Uswege M. Minga & John E. Olsen (2000) Molecular epidemiology of Salmonella gallinarum in chickens in Tanzania, Avian Pathology, 29:5, 457-463 en_US
dc.identifier.issn 0307-9457
dc.identifier.uri http://www.suaire.sua.ac.tz/handle/123456789/1391
dc.description Avian Pathology, 2000; 29:457– 463 en_US
dc.description.abstract A molecular epidemiological investigation of Salmonella gallinarum infection in scavenging local chickens and commercial layers in Tanzania was conducted between August 1997 and April 1998. A total of 1152 chickens were randomly selected from 10 villages and seven commercial farms. For serological and cultural prevalence studies, 1152 blood samples and 912 cloacal swabs were collected. In scavenging local chickens, the individual serological and cultural prevalences were 6.3 and 0%, while the prevalences were significantly higher in commercial layers at 18.4 and 2.6% (P < 0.001), respectively. The risk of infection in flocks of scavenging local chickens that had contact with commercial chickens was six times greater than the risk of infection in flocks of scavenging local chickens that had no contact with commercial chickens. Thirty-four S. gallinarum isolated from commercial chickens in this study, together with 29 Tanzanian historical isolates, were characterized using plasmid profiling and ribotyping. Fifty-one isolates contained both 85 and 2.5 kb plasmids, five isolates contained only one plasmid of 85 kb, and seven isolates had no plasmids. Ribotyping using HindIII restriction endonuclease demonstrated seven different ribotypes. Forty-seven isolates had similar results in both typing systems, suggesting they belonged to one clone. It is concluded that S. gallinarum infection in chickens in Tanzania is more prevalent in commercial layers than in scavenging local chickens. One strain of S. gallinarum from chickens first isolated in a Dar es Salaam hatchery was found to be common throughout the country. en_US
dc.language.iso en en_US
dc.subject Molecular epidemiology en_US
dc.subject Salmonella gallinarum en_US
dc.subject Chickens en_US
dc.subject Commercial layers en_US
dc.subject Local chickens en_US
dc.title Molecular epidemiology of Salmonella gallinarum in chickens in Tanzania en_US
dc.type Article en_US


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