Detection and quantification of aflatoxin b 1 in cultured Nile tilapia in Mvomero and Mbarali districts of Tanzania
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Date
2015
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Publisher
Sokoine University of Agriculture
Abstract
This study was carried out to assess farmers’ awareness as well as to detect and quantify
the levels of Aflatoxin B1 in Nile tilapia (Oreochromis niloticus) farms in Mbarali and
Mvomero districts. A total of 36 farmers and extension workers were involved in a
questionnaire survey. Ninety two samples, tilapia (n=62), fish feeds (n=10), organic
manure (n=10) and pond water (n=10) were collected in February 2013 and assessed for
Aflatoxin B1 by a competitive ELISA technique. About 44.4% of the respondents were
aware of Aflatoxin B1. This toxin was found in all samples collected although the levels
between the samples differed significantly (P < 0.05). Out of 92 samples collected, 7.6%
showed Aflatoxin B1 concentration greater than 5.0 μg/kg. Also, out of 62 fish samples
collected from both Districts, 98% were contaminated with Aflatoxin B1 while the
remaining fish samples had Aflatoxin B1 concentration greater than maximum tolerable
limit of 5.0 μg/kg set by TBS/FAO/WHO. The concentration of Aflatoxin B1 in manure
and fish samples was significantly higher in contamination (p= 0.016, and 0.001,
respectively at 95% C.I) in samples collected in Mvomero compared with those from
Mbarali District. In conclusion, most farmers were not aware of aflatoxins or of measures
to control the toxins in the field, which begins with good agricultural and handling
practices (GAP/GHP). Aflatoxin B1 levels in fish were below the internationally
acceptable limits for human consumption. The detection of small quantities suggest a
need for extensive mycotoxin awareness creation and education program as well as the
adoption of good agricultural practices that include proper storage and handling of feeds
in Mvomero and Mbarali Districts.
Description
MSc Thesis
Keywords
Mycotoxin awareness, Good agricultural practices, Aflatoxin quantification, Aflatoxin detection, Nile tilapia, Feed handling