Sokoine University of Agriculture

Molecular epidemiology of Leptospira species among Agropastoral communities living in Katavi-Rukwa ecosystem, Tanzania

Show simple item record Muller, Shabani Kililwa 2017-02-21T10:51:39Z 2017-02-21T10:51:39Z 2015
dc.description.abstract Leptospirosis is an emerging zoonotic infectious disease which affects humans and animals worldwide as it causes febrile illness in humans. The disease has been reported in a number of human-livestock-wildlife interfaces of Northern and Eastern Tanzania. Very little is known of many zoonotic disease conditions in the research naive areas of Western and Southern Tanzania. This study aimed at detecting the prevalence of Leptospira species among agro-pastoralists at the human-animal interface areas of Katavi-Rukwa ecosystem. Microscopic agglutination test (MAT) was used to detect antibody against six Leptospira antigens including local serogroups Icterohaemorrhagiae, Ballum, Grippotyphosa, Sejroe and reference serogroups Hebdomadis and Lora. Samples with MAT titers ≥ 1:160 were scored as positive while MAT titers between 1:20 and 1:80 were scored as exposed to Leptospira and absence of agglutination titers was scored as negative. Of the 267 samples tested 80 (30%) were positive, 57 (21.3%) were negative and 130 (4 8.6%) were exposed to leptospiral infection. Infection rate in adults was higher 51 (63.75%) compared to children 29 (36.25%), P<0.05. Circulating serogroups were; Hardjo (15.7%); Icterohaemorrhagiae (8.98%), Grippotyphosa (4.87%), Hebdomadis (3.37%), Australis (1.49%) and Ballum (1.12%). Samples that were positive or scored as exposed by MAT were further tested using polymerase chain reaction (PCR) targeting 16S ribosomal gene. Pathogenic Leptospira was detected in 33 (15.5%) out of 212 while no saprophytic Leptospira species was detected. Sequencing alignment based on 16S ribosomal gene suggested Leptospira interrogans, kirshinei and uncultured Leptospira clone species as circulating species among agro-pastoralists of Katavi-Rukwa ecosystem. These findings suggest that in the Katavi leptospirosis in man is likely acquired from environment, probably by indirect contact with contaminated water or soil. This study also revealed that serological diagnosis of leptospirosis should be considered in the diagnosis on non- malarial febrile illness in agro-pastoralists living in Katavi-Rukwa ecosystem, Tanzania. en_US
dc.description.sponsorship Southern Africa Center for Infectious Disease Surveillance (SACIDS) en_US
dc.language.iso en en_US
dc.publisher Sokoine University of Agriculture en_US
dc.subject Molecular epidemiology en_US
dc.subject Leptospira species en_US
dc.subject Agropastoral communities en_US
dc.subject Katavi en_US
dc.subject Tanzania en_US
dc.subject Leptospirosis en_US
dc.subject Febrile illness en_US
dc.title Molecular epidemiology of Leptospira species among Agropastoral communities living in Katavi-Rukwa ecosystem, Tanzania en_US
dc.type Thesis en_US

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