Development of eight polymorphic microsatellite markers in the Black and Rufous sengi, Rhynchocyon petersi
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Date
2014-09
Journal Title
Journal ISSN
Volume Title
Publisher
Conservation Genetics Resources
Abstract
The Black and Rufous sengi, Ryhnchocyon
petersi, is endemic to a limited range in East Africa. We
report the development of eight polymorphic microsatellites
using next generation sequencing technology. Eighteen
individuals from Zaraninge forest (Saadani National
Park, Tanzania) were genotyped. The number of alleles per
locus ranged from 2 to 6, while the observed and expected
heterozygosities varied from 0.17 to 0.82 and from 0.25 to
0.81, respectively. No locus deviated from Hardy–Weinberg
equilibrium. These microsatellite markers will be
useful tools to study the effect of habitat fragmentation on
the population genetic structure of R. petersi.
Description
MICROSATELLITE LETTERS
Keywords
Rhynchocyon petersi, Vulnerable afrotherian, Microsatellites, 454 Sequencing