Sokoine University of Agriculture

Genotypes of Hepatitis B virus among voluntary blood donors in Kinshasa, Democratic Republic of Congo

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dc.contributor.author Moloko, Patience Maindo Pati
dc.date.accessioned 2016-12-01T13:17:53Z
dc.date.available 2016-12-01T13:17:53Z
dc.date.issued 2015
dc.identifier.uri https://www.suaire.sua.ac.tz/handle/123456789/1051
dc.description.abstract Hepatitis B represents a major global health problem. Despite the high endemicity of hepatitis B in Sub-Saharan Africa, little is known about the epidemiology and genotypes of hepatitis B virus (HBV) in Africa. The few published studies that describe the genetic characteristics of HBV in the Democratic Republic of Congo (DRC) involved a very small adult population size. In the present study, epidemiological and molecular characteristics of HBV infection were studied among voluntary blood donors (n=582) at the National Blood Transfusion Centre in Kinshasa, DRC. After obtaining an informed consent, a questionnaire was administered to collect socio-demographic data, followed by blood collection. Sampled blood was screened for hepatitis B surface antigen (HBsAg) using enzyme-linked immunosorbent assay (ELISA). The prevalence of HBsAg among voluntary blood donors in Kinshasa was found to be 6.9 % (40/582). Blood donors aged between 28 and 37 years had the highest HBsAg prevalence. Using PCR targeting the partial S gene, HBV-DNA was detected in 50% (20/40) of HBsAg positive samples and afterwards sequenced in order to perform phylogenetic analysis. Nine (60.0 %), four (26.6 %) and one (6.7 %) out of the 15 HBV nucleotide sequences clustered into genotypes A, E and D, respectively. One (6.7 %) HBV nucleotide sequence did not cluster with any of the known genotypes and could be a novel genotype, subgenotype or recombinant HBV. The present study confirms that HBV infection is endemic in Kinshasa and demonstrates that HBV genotype A predominates in adults. Further studies are needed to assess detailed virological and clinical characteristics of HBV genotypes, as well as the existence of HBV recombinant genotypes in DRC, which may have an impact on clinical outcomes, drug effect and vaccination efficacy. en_US
dc.description.sponsorship Intra-Africa, Caribbean and Pacific regions (Intra-ACP) academic mobility scheme en_US
dc.language.iso en en_US
dc.publisher Sokoine University of Agriculture en_US
dc.subject blood donors in Kinshasa en_US
dc.subject Democratic Republic of Congo en_US
dc.subject Genotypes en_US
dc.subject Hepatitis B en_US
dc.title Genotypes of Hepatitis B virus among voluntary blood donors in Kinshasa, Democratic Republic of Congo en_US
dc.type Thesis en_US


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