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    Effects of albizia lebbeck on haematological parameters and histological structure of digestive organs in rats
    (Sokoine University of Agriculture, 2021) Gwakisa, Aron
    In Tanzania, Albizia lebbeck is among the multipurpose plant used for treatment of various diseases and as a fodder. However, scientific information on the effects of this plant in the animal body is non-existent. Therefore, this study was conducted to evaluate the effects of methanol extracts of stem barks (SB) and leaves of Albizia lebbeck on haematological parameters and histological structure of digestive organs in rats. Sixty rats were randomly allotted into ten groups (I-X) of six rats each. Methanol extracts of SB and leaves of A. lebbeck were administered orally at doses of 100 mg/kg and 400 mg/kg to the experimental groups and the control groups received distilled water orally for five and 30 days. On days six and 31, rats were sacrificed and blood samples were collected into tripotassium ethylenediamine tetraacetate tubes for haematological analysis and tissue samples from the digestive organs (liver, stomach and jejunum) were immersion-fixed in 10% neutral buffered formalin for histopathological evaluation. Results revealed that, after five days of treatment, both extracts of A. lebbeck significantly (P<0.05) increased thrombocytes and plateletcrit at 400 mg/kg, and platelets distribution width (PDW) at 400 mg/kg SB compared to the control group. After 30 days of treatment, both extracts significantly (P<0.05) increased leucocytes at 100 mg/kg, lymphocytes and eosinophils at 400 mg/kg, also eosinophils and haematocrit at 100 mg/kg leaves, and erythrocytes at 100 mg/kg SB compared to the control group. Both extracts significantly (P<0.05) decreased mean corpuscular volume, mean platelet volume, and PDW at 100 mg/kg, monocytes at 100 mg/kg SB, and monocytes and neutrophils at 400 mg/kg leaves compared to the control group. Other haematological parameters changed insignificantly (P>0.05) at both doses and treatment durations. Histopathological lesions in the liver including empty spaces in cytoplasm of hepatocytes, sinusoids compression, necrosis, and inflammatory cells infiltration were observed for both extracts. There was no significant histopathological lesion in the stomach and jejunum at both doses and treatment durations. Therefore, prolonged use of both extracts should be discouraged because may affects haematological parameters and the liver.