Browsing by Author "Mlay, J. D."
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Item Detection of Contagious bovine pleuropneumonia in condemned cattle lungs at Morogoro municipal abattoir in Tanzania(Tanzania Veterinary Journal, 2013) Malago, J. J.; Mlay, J. D.Control of re-emerged Contagious bovine pleuropneumonia (CBPP) in Tanzania in 1990s left spots of unvaccinated animals in various areas. Some of these animals were carriers of CBPP and have presumably continued to be sources of infection to other animals. We made an abattoir follow-up of slaughtered animals to understand whether the disease is still present in Tanzania. A total of 13 condemned lungs due to CBPP-like lesions at Morogoro municipal abattoir were collected from November 2011 to April 2012 and examined grossly, histologically and bacteriologically. Typical gross lesions of CBPP including expanded interlobular septa, sequestration, coalescing lungs, and fibrinonecrotic exudation were observed. Histologically, we observed fibrinonecrotic exudates filling and expanding the alveoli, desquamation of alveolar epithelial cells, lymphoplasmacytic infiltration in the interalveolar septa and around bronchi, bronchioles, and blood vessels, and vasculitis with subsequent vascular rupture and hemorrhage. Mycoplama cultures in two samples isolated Mycoplasma organisms with “fried egg appearance”, typical of Mycoplasma mycoides mycoides small colony type, the causative agent of CBPP. We conclude that CBPP is still prevalent in Tanzania and continues to pose a potential impending epidemic in the future.Item Detection of Contagious bovine pleuropneumonia in condemned cattle lungs at Morogoro municipal abattoir in Tanzania(Tanzania Veterinary Journal, 2013) Malago, J. J.; Mlay, J. D.Control of re-emerged Contagious bovine pleuropneumonia (CBPP) in Tanzania in 1990s left spots of unvaccinated animals in various areas. Some of these animals were carriers of CBPP and have presumably continued to be sources of infection to other animals. We made an abattoir follow-up of slaughtered animals to understand whether the disease is still present in Tanzania. A total of 13 condemned lungs due to CBPP-like lesions at Morogoro municipal abattoir were collected from November 2011 to April 2012 and examined grossly, histologically and bacteriologically. Typical gross lesions of CBPP including expanded interlobular septa, sequestration, coalescing lungs, and fibrinonecrotic exudation were observed. Histologically, we observed fibrinonecrotic exudates filling and expanding the alveoli, desquamation of alveolar epithelial cells, lymphoplasmacytic infiltration in the interalveolar septa and around bronchi, bronchioles, and blood vessels, and vasculitis with subsequent vascular rupture and hemorrhage. Mycoplama cultures in two samples isolated Mycoplasma organisms with “fried egg appearance”, typical of Mycoplasma mycoides mycoides small colony type, the causative agent of CBPP. We conclude that CBPP is still prevalent in Tanzania and continues to pose a potential impending epidemic in the future.Item Immunohistochemical detection of Mycoplasma mucoid mucoid small colony type in lungs of slaughtered cattle at Morogoro slaughterhouse, Tanzania(Tanzania Veterinary Journal, 2015) Malago, J. J.; Mlay, J. D.Diagnosis of contagious bovine pleuropneumonia (CBPP) in carrier animals remains to be a challenge in developing countries partly because of lack of diagnostic tools that can detect asymptomatic animals. As a result many apparently healthy animals pose a potential impending epidemic in periods of stress. We examined condemned lungs with CBPP-like lesions from apparently healthy slaughtered animals to confirm presence of the disease in these animals. A total of 13 lungs collected at abattoir were examined immunohistochemically using 3H12 and 6E3 monoclonal antibodies against Mycoplasma mycoides mycoides (MmmSC), the causative agent of CBPP. Both 3H12 and 6E3 antibodies detected the MmmSC antigens in all the 13 lungs in bronchial, bronchiolar and alveolar epithelia, bronchial glands, bronchial cartilage, and in alveoli. We conclude that CBPP is still present in various places of Tanzania, posing a threat to livestock production and that immunohistochemistry can be an efficient diagnostic method to confirm presence of the disease in asymptomatic healthy animals passed for slaughter.